PCATALOG
The Importance of the Retention Rate of Stationary Phase in HPCCC
Pubdate：2016-09-18 Click：1657
The Importance of the Retention Rate of Stationary Phase in HPCCC
HPCCC is a new technique of liquid chromatography,using liquid-to-liquid of two-phase counter current distribution, in the absence of solid fillers, without the use of solid stationary phase, under the condition of constant force. The use of the centrifugal force generated by the retention of the stationary phase in a series connected pipeline cavity realize the separation of complex chemical mixtures materials. It takes the liquid solvent as the stationary phase, the centrifugal force generated in the planetary motion spiral column, making the immiscible two-phase continuous mix with each other. While retaining a phase in which using the constant flow pump continuous input in another phase, and the solute distribution between two phases in the samples repeatedly, in terms of the distribution coefficient of the order was eluted come out, it replaces the traditional packed column preparative high performance liquid chromatography.
Dynamic HPCCC in the operation process, two-dimensional direction column coil in the revolution and rotation of the rotating speed, the centrifugal force remained very high, "G", and IT revolution radius and revolution radius, rotation speed and other relevant factors, the purpose of which is to maintain a stationary phase in the column in the coil, and the mobile phase was not launched "G" force ultimately reflected in the retention of stationary phase, the stationary phase retention rate is the most important indicator of a HPCCC, the stationary phase retention in the instrument factory and the installation process can be determined; the constant flow pump in low speed rotation the case will be packed into the column in the coil, and then rotating at high speed under the condition of the mobile phase was injected into the chromatographic column in the coil, is measured by the launch of the stationary phase volume, the retention of stationary phase (= The volume of the chromatographic column, the volume of the stationary phase, and the volume of the column coil, which it’s a comprehensive performance index of the instrument, directly determine the degree of separation in the process of sample purification.
The British AECS company is the world leader in HSCCC&HPCCC, the only thing in the world to achieve an instrument configuration of 1 group, 2 group, 4 group chromatography coi. The Coil can choose PTFE, Ti stainless steel, alloy material, in order to adapt to the different pressure, different pH and other special requirements, is the only one in the world at the same time with and production of static HPCPC and dynamic HSCCC manufacturers, the HSCCC stationary phase retention rate is more than 95%, the test report and on-site test results as shown below. The British company AECS purification by high speed countercurrent color spectrometer in the world widely used in the field of chemistry, such as antibiotics, shrinkage ammonia acid, tannin, saponin, grease, chiral drugs, natural products, food additives, flavors and fragrances, oil chemical additives etc..
Goodwill (Hongkong) Technology Co., Ltd as general agent of HPCCC of the British AECS company in China, with fully responsible for the sales of its products and technology: 010-66155031/32/33.