PCATALOG
Extraction of Dioxin and other Semi-volatile Organic Compounds (SVOC) in Food
Pubdate：2021-03-08 Click：913
AN number: LA1020
Date: 2017-02-27
Revision:1.0
As the extraction might involve the use of hazardous and hot solvent it is strongly recommended to use protective glasses and gloves. The SoxROC Operation Manual chapter 3 Safety should be read before starting any work.
INTRODUCTION
This application note addresses the extraction of matters for further gas chromatographic (GC) analysis of Dioxin and other semi-volatile organic compounds (SVOC) in food. Typical samples is meat, pork, fish and chicken.
Soxhlet and hot solvent extraction are the most common extraction techniques for solid samples. GC analysis typically require minimum 3-4g fat extracted from the sample.
EXPERIMENTAL
Apparatus
▲Scissors
▲Analytical balance
▲Drying oven
▲Desiccator
▲ SoxROC Extraction Unit SX-360-A or SX-320-A
★Extraction cups φ54 mm, aluminum, compatible with the solvent extraction system, allowing lower boiling temperature
★Specific Large Cellulose thimbles (40x80 mm)
★Specific Sealing rings in Butyl to handle Acetone Solvent
Reagents and accessories
▲Acetone suitable for samples such as Dioxin in Food
▲Boiling stones
▲Cotton woo
Preparation
A large cellulose thimble (40x80mm) from OPSIS LiquidLINE can contain up to 50g sample, whereas the standard thimble (33x80 mm) typically only fits 30g. This means that samples with 6% and more can be extracted with the SoxROC. However, it is anyhow advisable to dry freeze the samples prior to extraction if the samples contain less than 10% fat, mostly due to risk with sampling errors.
In case there is a concern for contamination then Glass Microfiber Thimbles can be used instead of Cellulose thimbles. Glass microfiber thimbles can be heated in an oven at 550°C. to remove contamination from other organic substances.
Procedure
Weigh the sample into a thimble and place a piece of cotton wool on top of the sample.Carry out the extraction with SoxROC Extraction Unit using the parameters shown in Table 1Dry the extracts to constant weight in an oven at 100°C for 60 minutes and let cool to room temperature in desiccator.
Continue analysis with GC.
Table 1. Extraction parameters (SoxROC Extraction Unit)
|
SoxROC configuration |
Butyl Seals (SX-035-A) for Acetone solvents
Aluminum cup (SX-023-A) |
|
Sample weight |
Depending on thimble and fat content. Max 500 ample in a 40x80 mm thimble, but less is recommended for best results |
|
Solvent |
Acetone |
|
Solvent volume |
100-150 ml |
|
Temperature |
200℃ (hiaher if Glass cup is used) |
|
Boiling /number of reduces |
30 min/4 |
|
Rinsing/number of reduces |
60 min/5 |
|
Drying |
5 min |
REFERENCES
*This Application Note should be used in conjunction with Application Note LA1002 "Application Guide SoxROC Solvent Extraction"
*OG1012 SoxROC Operation Guide
*ISO 16215:2012: Animal feeding stuffs. Determination of dioxins and dioxin-like PCBs by GC/HRMS and of indicator PCBs by GC/HRMS
*Commission Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs.