PCATALOG
Impact of trehalose on physicochemical stability of β-carotene high loaded microcapsules fabricated by wet-milling coupled with spray drying
Pubdate：2022-01-13 Click：864
Solvent-free p-carotene high loaded microcapsules formulated by octenyl succinic anhydride (OSA)-starch and rehalose were prepared by a combination of wet-milling and spray drying. The influence of different mass ratios of OSA-starch to trehalose on the particle size distribution, storage stability, hygroscopicity and redispersibility of p-carotene microcapsules was investigated. The optimized microcapsules exhibited a small average diamete: (228.2 nm) after rehydration with high encapsulation efficiency and loading capacity (99.06% and 10.32% respectively). The phase separation in the OSA-starch and trehalose composite matrix was observed in the presence of trehalose at a high level, which was favorable for the prominent storage stability of p-carotene . (96.16% for 50 days). The half-life of rehydrated p-carotene microcapsules was also extended by 2.23-and 1.25 olds during the thermal- and photo-degradation treatments. The crystallinity of p-carotene microcapsules was largely reduced due to the enhanced hydrogen bonding interaction between OSA-starch and p-carotene as analyzed by FTIR. SEM images showed that the morphology of p-carotene microcapsules was regulated by the level of trehalose. Confocal Raman spectrum microscopy and hygroscopic variation jointly verified the entrapment of p-carotene into a glassy matrix formed at a high level of trehalose. Moreover, a high level of trehalose could improve the spontaneous sinkability and dispersibility of p-carotene microcapsules, which were completely rehydrated in less than 1 min.
ConclusionIn
this study, p-carotene high loaded microcapsules were successfully fabricated by wet-milling coupled with spray drying. Trehalose acted as a glassy matrix former or hydroxyl groups supplier in the formation of B-carotene microcapsules. As the level of trehalose was increased, the physicochemical stability of both p-carotene microcapsule powders and rehydrated dispersions was obviously improved. The incorporation of trehalose was favorable to preventing p-carotene nanocrystals from agglutination during spray drying due to the enhancement of hydrogen bonding interactions between p-carotene and OSA-starch. The phase separation of the wall material occurred in the presence of trehalose at a high level, which could effectively prevent oxygen from penetration, The reduced free volume of the p-carotene microcapsules was responsible for their prominent storage stability since less oxygen could be encased inside the microcapsules. The stability of both p-carotene nanosuspensions and rehydrated microcapsules was also increased due to the reduction in the solubility and diffusivity of oxygen in the presence of more hydroxyl groups. Furthermore, the microcapsules formulated with a high level of trehalose exhibited the instant dissolution property due to the enhanced wettability and the existence of planetary agglomerations.