PCATALOG
Preparation and Characterization of Enzymatic Hydrolysis of Duck Blood Corpuscle Short Peptid
Pubdate：2024-01-10 Click：435
Abstract：The aims of this study were to screen the commercial protease with high hydrolysis eficiency and good decolorization effect, and to establish process technology of blood corpuscle short peptide, compared the nutritional characteristics before and aftere nzymatic hydrolysis, to study its nutritional value, functional properties and antioxidant activity in vivo. The functional product of blood corpuscle short peptide was produced so as to offer theoretic basis and technical reference for the eficient conversion and utilization of poultry blood. Tak- ing degree of hydrolysis(DH), decoloration and hydrolysate yield as criterion, the effect of main parameters (such as types of protease, enzyme dosage, temperature, pH and hydrolysis time)and the optimal hydrolysis conditions were established based on the method of single-factor and orthogonal experiments. The blood corpuscle short peptide was used to study its nutritional, functional properties and antioxidant activity. We selected the acidic protease as the best enzyme to hydrolyze protein in our experiments. The suitable conditions of enzymatic hydrolysis, i.e. Enzyme dosage 6000U/g, hydrolysis temperature 50℃, pH3.5, hydrolysis time 7.0h, and the degree of hydrolysis was(25.10±0.65)％hydrolysate yield was(60.09±1.77)％under the optimal conditions. The molecular weight distribution of the hydrolysate as determined by high performance liquid chromatography (HPLC) suggested that significant degradation of blood corpuscle proteins produced the hydrolysate mostly consisting of short peptides below 3ku with molecules less than 1ku accounting for the majority (62.82％) of the total peptides. The white blood corpuscle short peptide powder containing all the common amino acids was rich in essential amino acids(53.31％) and had excellent solubility (>60％)and emulsifying stability. With the increasing concentration of blood corpuscleprotein, its free radical scavenging activity [1,1- diphenyl-2-picryl hydrazyl radical 2, 2-diphenyl-1-(2,4,6-trinitropheny1) hydrazyl (DPPH· )and superoxide anion] and reducing power increased. Therefore acid protease serves best for the hydrolysis of duck blood corpuscle protein, and its peptide powder has good features with high protein, rich essential amino acids and strong antioxidant activity. The blood corp uscle short peptide canbeutilized as functional material for the food and feed in dustry. [Chinese Journal of Animal Nutrition, 2016, 28(8): 2521-2533]
Key words: blood corpuscle short peptide; acid protease; functional property; antioxidant activity
